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3d insight human islet microtissues  (Insphero Inc)

 
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    Structured Review

    Insphero Inc 3d insight human islet microtissues
    3d Insight Human Islet Microtissues, supplied by Insphero Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+insight+human+islet+microtissues/3d+insight+human+islet+microtissues/pm40645444-79-9-8
    Average 90 stars, based on 1 article reviews
    3d insight human islet microtissues - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Pancreatic islet organoids and organoids on-chip for type 1 diabetes.
    Article Snippet: .. In this way, an alternative is proposed with InSphero 3D InSight human islet microtissues source obtained by scaffold-free reaggregation of dispersed primary human islets, using a hanging-drop method (Table 2) and cultured according to InSphero’s instructions [31]. ..

    other:

    Article Title: A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets
    Article Snippet: 3D InSight Human Islet Microtissues were obtained from InSphero AG (Schlieren, Switzerland) and maintained in 3D InSight Human Islet Maintenance Medium, also from InSphero AG (Schlieren, Switzerland) at 37°C and 5% CO 2 .

    Gentle:

    Article Title: Targeted pharmacological therapy restores β-cell function for diabetes remission.
    Article Snippet: .. For each preparation of InSphero 3D InSight human islet microtissues, 10,000–20,000 islet equivalents were dispersed in dissociation solution (1× TrypLE Express solution (Thermo Fisher Scientific 12604013) with 40 μg ml−1 DNase I (Sigma-Aldrich 10104159001)) by gentle pipetting at 37 °C. ..

    Produced:

    Article Title: Human Islet Microtissues as an In Vitro and an In Vivo Model System for Diabetes
    Article Snippet: .. InSphero 3D InSight human islet microtissues (InSphero AG, Schlieren, Switzerland) were produced by hanging-drop-based scaffold-free reaggregation of dispersed primary human islets obtained from Prodo Laboratories Inc. (Irvine, CA, USA). ..

    Article Title: Upregulation of HLA class II in pancreatic beta cells from organ donors with type 1 diabetes.
    Article Snippet: Aims/hypothesis We aimed to characterise and quantify the expression of HLA class II (HLA-II) in human pancreatic tissue sections and to analyse its induction in human islets.. Methods We immunostained human pancreatic tissue sections from non-diabetic (n = 5), autoantibody positive (Aab+; n = 5), and type 1 diabetic (n = 5) donors, obtained from the Network of Pancreatic Organ Donors (nPOD), with HLA-II, CD68 and insulin.. Each tissue section was acquired with a widefield slide scanner and then analysed with QuPath software.

    Isolation:

    Article Title: Upregulation of HLA class II in pancreatic beta cells from organ donors with type 1 diabetes.
    Article Snippet: Aims/hypothesis We aimed to characterise and quantify the expression of HLA class II (HLA-II) in human pancreatic tissue sections and to analyse its induction in human islets.. Methods We immunostained human pancreatic tissue sections from non-diabetic (n = 5), autoantibody positive (Aab+; n = 5), and type 1 diabetic (n = 5) donors, obtained from the Network of Pancreatic Organ Donors (nPOD), with HLA-II, CD68 and insulin.. Each tissue section was acquired with a widefield slide scanner and then analysed with QuPath software.

    In Vitro:

    Article Title: Protection of pancreatic islets from oxidative cell death by a peripherally-active morphinan with increased drug safety.
    Article Snippet: .. 3D InSight human islet microtissues 3D InSight Human Islet Microtissues were provided by InSphero (MT-04-002-01) and represent an in vitro human islet model for diabetes research as well as an alternative to primary human pancreatic islets. ..



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    a) Stages of islet microtissue production displayed through immunofluorescence staining for insulin (β-cells, green), glucagon (α-cells, red), and somatostatin (δ-cells, blue). Images show (1) native islets in the human pancreas, (2) dissociated islets, and (3) islet <t>microtissues.</t> Scale bar: 100 μm. b) Endocrine-cell compositions of the islet microtissues were calculated from morphometric analysis of the microtissue sections from four individual donors. c) Cross-sectional diameter, d) total ATP content, e) total insulin content, and f) GSIS of human islet microtissues were assessed on days 7, 14, 21, and 28 post production. g) Glucose-stimulated insulin secretion of islet microtissues from 15 different donors 7 d post production is shown. ATP content, insulin content, and secreted insulin were normalized to the islet size in islet equivalents (IEQs), which refer to an islet with 150 μm diameter. Data in panels (c), (d), and (f) have been obtained from islets of seven individual donors, while panel (e) includes data of islets from four individual donors, mean ± standard deviations.
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    a) Stages of islet microtissue production displayed through immunofluorescence staining for insulin (β-cells, green), glucagon (α-cells, red), and somatostatin (δ-cells, blue). Images show (1) native islets in the human pancreas, (2) dissociated islets, and (3) islet <t>microtissues.</t> Scale bar: 100 μm. b) Endocrine-cell compositions of the islet microtissues were calculated from morphometric analysis of the microtissue sections from four individual donors. c) Cross-sectional diameter, d) total ATP content, e) total insulin content, and f) GSIS of human islet microtissues were assessed on days 7, 14, 21, and 28 post production. g) Glucose-stimulated insulin secretion of islet microtissues from 15 different donors 7 d post production is shown. ATP content, insulin content, and secreted insulin were normalized to the islet size in islet equivalents (IEQs), which refer to an islet with 150 μm diameter. Data in panels (c), (d), and (f) have been obtained from islets of seven individual donors, while panel (e) includes data of islets from four individual donors, mean ± standard deviations.
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    Image Search Results


    a) Stages of islet microtissue production displayed through immunofluorescence staining for insulin (β-cells, green), glucagon (α-cells, red), and somatostatin (δ-cells, blue). Images show (1) native islets in the human pancreas, (2) dissociated islets, and (3) islet microtissues. Scale bar: 100 μm. b) Endocrine-cell compositions of the islet microtissues were calculated from morphometric analysis of the microtissue sections from four individual donors. c) Cross-sectional diameter, d) total ATP content, e) total insulin content, and f) GSIS of human islet microtissues were assessed on days 7, 14, 21, and 28 post production. g) Glucose-stimulated insulin secretion of islet microtissues from 15 different donors 7 d post production is shown. ATP content, insulin content, and secreted insulin were normalized to the islet size in islet equivalents (IEQs), which refer to an islet with 150 μm diameter. Data in panels (c), (d), and (f) have been obtained from islets of seven individual donors, while panel (e) includes data of islets from four individual donors, mean ± standard deviations.

    Journal: Advanced biosystems

    Article Title: In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution

    doi: 10.1002/adbi.201900291

    Figure Lengend Snippet: a) Stages of islet microtissue production displayed through immunofluorescence staining for insulin (β-cells, green), glucagon (α-cells, red), and somatostatin (δ-cells, blue). Images show (1) native islets in the human pancreas, (2) dissociated islets, and (3) islet microtissues. Scale bar: 100 μm. b) Endocrine-cell compositions of the islet microtissues were calculated from morphometric analysis of the microtissue sections from four individual donors. c) Cross-sectional diameter, d) total ATP content, e) total insulin content, and f) GSIS of human islet microtissues were assessed on days 7, 14, 21, and 28 post production. g) Glucose-stimulated insulin secretion of islet microtissues from 15 different donors 7 d post production is shown. ATP content, insulin content, and secreted insulin were normalized to the islet size in islet equivalents (IEQs), which refer to an islet with 150 μm diameter. Data in panels (c), (d), and (f) have been obtained from islets of seven individual donors, while panel (e) includes data of islets from four individual donors, mean ± standard deviations.

    Article Snippet: Reaggregated Human Islets InSphero 3D InSight human islet microtissues were produced by hanging-drop-based scaffold-free reaggregation of dispersed primary human islets obtained through Prodo Laboratories Inc. Irvine, CA.

    Techniques: Immunofluorescence, Staining

    a) Stages of islet microtissue production displayed through immunofluorescence staining for insulin (β-cells, green), glucagon (α-cells, red), and somatostatin (δ-cells, blue). Images show (1) native islets in the human pancreas, (2) dissociated islets, and (3) islet microtissues. Scale bar: 100 μm. b) Endocrine-cell compositions of the islet microtissues were calculated from morphometric analysis of the microtissue sections from four individual donors. c) Cross-sectional diameter, d) total ATP content, e) total insulin content, and f) GSIS of human islet microtissues were assessed on days 7, 14, 21, and 28 post production. g) Glucose-stimulated insulin secretion of islet microtissues from 15 different donors 7 d post production is shown. ATP content, insulin content, and secreted insulin were normalized to the islet size in islet equivalents (IEQs), which refer to an islet with 150 μm diameter. Data in panels (c), (d), and (f) have been obtained from islets of seven individual donors, while panel (e) includes data of islets from four individual donors, mean ± standard deviations.

    Journal: Advanced biosystems

    Article Title: In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution

    doi: 10.1002/adbi.201900291

    Figure Lengend Snippet: a) Stages of islet microtissue production displayed through immunofluorescence staining for insulin (β-cells, green), glucagon (α-cells, red), and somatostatin (δ-cells, blue). Images show (1) native islets in the human pancreas, (2) dissociated islets, and (3) islet microtissues. Scale bar: 100 μm. b) Endocrine-cell compositions of the islet microtissues were calculated from morphometric analysis of the microtissue sections from four individual donors. c) Cross-sectional diameter, d) total ATP content, e) total insulin content, and f) GSIS of human islet microtissues were assessed on days 7, 14, 21, and 28 post production. g) Glucose-stimulated insulin secretion of islet microtissues from 15 different donors 7 d post production is shown. ATP content, insulin content, and secreted insulin were normalized to the islet size in islet equivalents (IEQs), which refer to an islet with 150 μm diameter. Data in panels (c), (d), and (f) have been obtained from islets of seven individual donors, while panel (e) includes data of islets from four individual donors, mean ± standard deviations.

    Article Snippet: Reaggregated Human Islets InSphero 3D InSight human islet microtissues were produced by hanging-drop-based scaffold-free reaggregation of dispersed primary human islets obtained through Prodo Laboratories Inc. Irvine, CA.

    Techniques: Immunofluorescence, Staining